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cell proliferation analysis gc 2spd ts cells  (Dojindo Labs)


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    Dojindo Labs cell proliferation analysis gc 2spd ts cells
    Effect of down-regulating MRE11 and RAD50 on cell proliferation and apoptosis of <t>GC-2spd(ts)</t> cells. a Down-regulation of MRE11 and RAD50 by transfecting GC-2spd(ts) cells with siRNA was analyzed through western blotting using GAPDH as the loading control. b Proliferation of GC-2spd(ts) cells after transfection with MRE11 or RAD50 siRNA was measured by the CCK8 assay. Apoptosis of GC-2spd(ts) cells after siRNA transfection for 72 h was analyzed by c flow cytometry and d TUNEL assays. The scale bars represent 40 μm. Each assay was performed in triplicate. Data are presented as means ± SD. *P < 0.05.
    Cell Proliferation Analysis Gc 2spd Ts Cells, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 60488 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/analysis+ts/pmc07056783-97-0-41
    Average 99 stars, based on 60488 article reviews
    cell proliferation analysis gc 2spd ts cells - by Bioz Stars, 2026-10
    99/100 stars

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    1) Product Images from "Decreased expression of MRE11 and RAD50 in testes from humans with spermatogenic failure"

    Article Title: Decreased expression of MRE11 and RAD50 in testes from humans with spermatogenic failure

    Journal: Journal of Assisted Reproduction and Genetics

    doi: 10.1007/s10815-019-01686-5

    Effect of down-regulating MRE11 and RAD50 on cell proliferation and apoptosis of GC-2spd(ts) cells. a Down-regulation of MRE11 and RAD50 by transfecting GC-2spd(ts) cells with siRNA was analyzed through western blotting using GAPDH as the loading control. b Proliferation of GC-2spd(ts) cells after transfection with MRE11 or RAD50 siRNA was measured by the CCK8 assay. Apoptosis of GC-2spd(ts) cells after siRNA transfection for 72 h was analyzed by c flow cytometry and d TUNEL assays. The scale bars represent 40 μm. Each assay was performed in triplicate. Data are presented as means ± SD. *P < 0.05.
    Figure Legend Snippet: Effect of down-regulating MRE11 and RAD50 on cell proliferation and apoptosis of GC-2spd(ts) cells. a Down-regulation of MRE11 and RAD50 by transfecting GC-2spd(ts) cells with siRNA was analyzed through western blotting using GAPDH as the loading control. b Proliferation of GC-2spd(ts) cells after transfection with MRE11 or RAD50 siRNA was measured by the CCK8 assay. Apoptosis of GC-2spd(ts) cells after siRNA transfection for 72 h was analyzed by c flow cytometry and d TUNEL assays. The scale bars represent 40 μm. Each assay was performed in triplicate. Data are presented as means ± SD. *P < 0.05.

    Techniques Used: Western Blot, Control, Transfection, CCK-8 Assay, Flow Cytometry, TUNEL Assay

    Related Articles

    Cell Culture:

    Article Title: Decreased expression of MRE11 and RAD50 in testes from humans with spermatogenic failure
    Article Snippet: .. Cell proliferation analysis GC-2spd(ts) cells were seeded and cultured in triplicate for each group at 3 × 10 3 cells/well in 96-well plates and reached 60–70% confluence after 24 h. Cell proliferation was determined using the Cell Counting Kit-8 (CCK-8) assay (Dojindo Molecular Technologies, Shanghai, China) according to the manufacturer’s protocol at 24, 48, and 72 h after siRNA transfection. .. In brief, 10 μL of the CCK-8 solution were added into each well and the absorbance was measured at 450 nm after 2 h of incubation at 37 °C using a spectrophotometer reader (Thermo Fisher Scientific).

    CCK-8 Assay:

    Article Title: Decreased expression of MRE11 and RAD50 in testes from humans with spermatogenic failure
    Article Snippet: .. Cell proliferation analysis GC-2spd(ts) cells were seeded and cultured in triplicate for each group at 3 × 10 3 cells/well in 96-well plates and reached 60–70% confluence after 24 h. Cell proliferation was determined using the Cell Counting Kit-8 (CCK-8) assay (Dojindo Molecular Technologies, Shanghai, China) according to the manufacturer’s protocol at 24, 48, and 72 h after siRNA transfection. .. In brief, 10 μL of the CCK-8 solution were added into each well and the absorbance was measured at 450 nm after 2 h of incubation at 37 °C using a spectrophotometer reader (Thermo Fisher Scientific).

    Transfection:

    Article Title: Decreased expression of MRE11 and RAD50 in testes from humans with spermatogenic failure
    Article Snippet: .. Cell proliferation analysis GC-2spd(ts) cells were seeded and cultured in triplicate for each group at 3 × 10 3 cells/well in 96-well plates and reached 60–70% confluence after 24 h. Cell proliferation was determined using the Cell Counting Kit-8 (CCK-8) assay (Dojindo Molecular Technologies, Shanghai, China) according to the manufacturer’s protocol at 24, 48, and 72 h after siRNA transfection. .. In brief, 10 μL of the CCK-8 solution were added into each well and the absorbance was measured at 450 nm after 2 h of incubation at 37 °C using a spectrophotometer reader (Thermo Fisher Scientific).



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    Dojindo Labs cell proliferation analysis gc 2spd ts cells
    Effect of down-regulating MRE11 and RAD50 on cell proliferation and apoptosis of <t>GC-2spd(ts)</t> cells. a Down-regulation of MRE11 and RAD50 by transfecting GC-2spd(ts) cells with siRNA was analyzed through western blotting using GAPDH as the loading control. b Proliferation of GC-2spd(ts) cells after transfection with MRE11 or RAD50 siRNA was measured by the CCK8 assay. Apoptosis of GC-2spd(ts) cells after siRNA transfection for 72 h was analyzed by c flow cytometry and d TUNEL assays. The scale bars represent 40 μm. Each assay was performed in triplicate. Data are presented as means ± SD. *P < 0.05.
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    Image Search Results


    Effect of down-regulating MRE11 and RAD50 on cell proliferation and apoptosis of GC-2spd(ts) cells. a Down-regulation of MRE11 and RAD50 by transfecting GC-2spd(ts) cells with siRNA was analyzed through western blotting using GAPDH as the loading control. b Proliferation of GC-2spd(ts) cells after transfection with MRE11 or RAD50 siRNA was measured by the CCK8 assay. Apoptosis of GC-2spd(ts) cells after siRNA transfection for 72 h was analyzed by c flow cytometry and d TUNEL assays. The scale bars represent 40 μm. Each assay was performed in triplicate. Data are presented as means ± SD. *P < 0.05.

    Journal: Journal of Assisted Reproduction and Genetics

    Article Title: Decreased expression of MRE11 and RAD50 in testes from humans with spermatogenic failure

    doi: 10.1007/s10815-019-01686-5

    Figure Lengend Snippet: Effect of down-regulating MRE11 and RAD50 on cell proliferation and apoptosis of GC-2spd(ts) cells. a Down-regulation of MRE11 and RAD50 by transfecting GC-2spd(ts) cells with siRNA was analyzed through western blotting using GAPDH as the loading control. b Proliferation of GC-2spd(ts) cells after transfection with MRE11 or RAD50 siRNA was measured by the CCK8 assay. Apoptosis of GC-2spd(ts) cells after siRNA transfection for 72 h was analyzed by c flow cytometry and d TUNEL assays. The scale bars represent 40 μm. Each assay was performed in triplicate. Data are presented as means ± SD. *P < 0.05.

    Article Snippet: Cell proliferation analysis GC-2spd(ts) cells were seeded and cultured in triplicate for each group at 3 × 10 3 cells/well in 96-well plates and reached 60–70% confluence after 24 h. Cell proliferation was determined using the Cell Counting Kit-8 (CCK-8) assay (Dojindo Molecular Technologies, Shanghai, China) according to the manufacturer’s protocol at 24, 48, and 72 h after siRNA transfection.

    Techniques: Western Blot, Control, Transfection, CCK-8 Assay, Flow Cytometry, TUNEL Assay